Research of interaction of fluorescent anti-CD133 DNA aptamers with glioblastoma cell cultures using flow cytometry

Key Points

• This research investigates how fluorescent DNA aptamers identify the CD133 marker in glioblastoma cells.
• Used fluorescein-labeled DNA aptamers of the Cs and Ap series.
• Applied these aptamers at a concentration of 1 μM.
• Employed flow cytometry to analyze the binding of aptamers to cells.
• Successfully detected CD133 marker in glioblastoma cells.
• The method allows for continuous monitoring of cell cultures.