This research investigates how fluorescent DNA aptamers identify the CD133 marker in glioblastoma cells.
Used fluorescein-labeled DNA aptamers of the Cs and Ap series.
Applied these aptamers at a concentration of 1 μM.
Employed flow cytometry to analyze the binding of aptamers to cells.
Successfully detected CD133 marker in glioblastoma cells.
The method allows for continuous monitoring of cell cultures.
Abstract
Fluorescein-labeled DNA aptamers of the Cs and Ap series at a concentration of 1 μM enable detection of the CD133 marker in continuous cell cultures of GB through flow cytometry.