The Small Molecule Compound Eupalinolide B Ameliorates Depressive Behaviors and Neuropathic Pain in Mice With Spared Nerve Injury: Integrating Network Pharmacology, Molecular Docking, Bioinformatics, Molecular Dynamics Simulation and Experimental Verification

Key Points

• The aim is to explore how eupalinolide B alleviates depressive behaviors and neuropathic pain in mice.
• Utilized network pharmacology to identify molecular targets.
• Performed molecular docking to investigate binding interactions.
• Conducted bioinformatics analyses for protein expression profiles.
• Applied molecular dynamics simulations to assess stability of complexes.
• Validated findings through experimental verification in animal models.
• Eupalinolide B reduced expression levels of EGFR and PTGS2 proteins.
• Improved synaptic plasticity in the observed mice.
• Alleviated both depressive behaviors and neuropathic pain caused by nerve injury.