A Zwitterionic-Enabled One-Pot Electrochemical Strategy for Stable End-Point Detection of Isothermal Nucleic Acid Amplification

Key Points

• This research aims to develop a stable electrochemical strategy for detecting nucleic acid amplification.
• Utilized a zwitterionic-enabled one-pot strategy in conjunction with loop-mediated isothermal amplification.
• Focus on minimizing interfacial fouling to improve signal stability.
• Developed an indicator-based electrochemical detection system.
• Achieved significant improvements in signal stability during nucleic acid amplification.
• Demonstrated successful application in portable nucleic acid testing.