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September 1, 1971Proceedings of the National Academy of Sciences24 citationsOpen Access

The 3′-Terminal Nucleosides of the High Molecular Weight RNA of C-Type Viruses

HMHideto MaruyamaHokkaido UniversityMHMasakazu HatanakaKagoshima UniversityRGRaymond V. GildenMeadowlark Optics (United States)

Key Result

Analysis of tumor virus RNAs from several mammalian and one reptilian species revealed that each virus contained uridine as the predominant 3'-terminal nucleoside.

PICO

P
Population
C-Type Viruses
I
Intervention / Comparator
Terminal tritiation and hydrolysis
O
Primary Outcome
3'-terminal nucleoside identification and molecular weight estimation

Limitations

  • Some nonspecific breakdown of the viral RNA occurs during the treatment to expose new termini, leading to variability in the percent activity seen with other nucleosides.

Abstract

Tumor virus RNAs from several mammalian and one reptilian species were purified; their 3'-terminal nucleoside was identified by separation of the trialcohols produced by periodate oxidation followed by reduction and tritiation with NaB(3)H(4). Each virus contained uridine as the predominant terminal nucleoside. Molecular weight estimations based on the tritiation reactions were consistent with a structure consisting of four subunits.

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Cite This Study

Maruyama et al. (1971) studied C-Type Viruses. Terminal tritiation and hydrolysis was evaluated on 3'-terminal nucleoside identification and molecular weight estimation. Analysis of tumor virus RNAs from several mammalian and one reptilian species revealed that each virus contained uridine as the predominant 3'-terminal nucleoside.

synapsesocial.com/papers/6a92a60fd3678382206234e0https://doi.org/10.1073/pnas.68.9.1999
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