PulseExploreJournal ClubDebatesTrendingResearchersJournals
Instagram
HomeExploreJournal ClubTrending
Synapse
⌘+K
Synapse
September 1, 1971Proceedings of the National Academy of Sciences24 citationsOpen Access

The 3′-Terminal Nucleosides of the High Molecular Weight RNA of C-Type Viruses

HMHideto MaruyamaMHMasakazu HatanakaRGRaymond V. Gilden

Key Result

Analysis of tumor virus RNAs from several mammalian and one reptilian species revealed that each virus contained uridine as the predominant 3'-terminal nucleoside.

PICO

P
Population
C-Type Viruses
I
Intervention / Comparator
Terminal tritiation and hydrolysis
O
Primary Outcome
3'-terminal nucleoside identification and molecular weight estimation

Limitations

  • Some nonspecific breakdown of the viral RNA occurs during the treatment to expose new termini, leading to variability in the percent activity seen with other nucleosides.

Abstract

Tumor virus RNAs from several mammalian and one reptilian species were purified; their 3'-terminal nucleoside was identified by separation of the trialcohols produced by periodate oxidation followed by reduction and tritiation with NaB(3)H(4). Each virus contained uridine as the predominant terminal nucleoside. Molecular weight estimations based on the tritiation reactions were consistent with a structure consisting of four subunits.

Ask AI
Helpful
Bookmark
Share
View Full Paper

Cite This Study

Maruyama et al. (1971) studied C-Type Viruses. Terminal tritiation and hydrolysis was evaluated on 3'-terminal nucleoside identification and molecular weight estimation. Analysis of tumor virus RNAs from several mammalian and one reptilian species revealed that each virus contained uridine as the predominant 3'-terminal nucleoside.

synapsesocial.com/papers/6a92a60fd3678382206234e0https://doi.org/10.1073/pnas.68.9.1999
Ask AI
Helpful
Bookmark
Share
View Full Paper

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The 5′-Terminal Nucleotide Sequences of the Double-Stranded RNA of Human Reovirus1974 · 49 citations
  2. 2Determination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA1979 · 90 citations
  3. 3Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA1996 · 453 citations
  4. 4Nuclear Magnetic Resonance Study of RNA Structures at the 3′-End of the Hepatitis C Virus Genome2017 · 15 citations
  5. 5Towards identification of<i>cis</i>-acting elements involved in the replication of enterovirus and rhinovirus RNAs: a proposal for the existence of tRNA-like terminal structures1992 · 132 citations