Identification of a highly conserved sequence element at the 3' terminus of hepatitis C virus genome RNA
Key result
The hepatitis C virus genome RNA terminates with a highly conserved novel sequence of 98 bases, including a 46-base stable stem-loop structure, likely required for authentic HCV replication.
Population
Hepatitis C virus (HCV) genome RNA
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Refines HCV 3' terminus model; leaves open functional roles in replication for targeted studies.
Structured PICO
P
Population
Hepatitis C virus (HCV) genome RNA
I
Intervention
Sequence analysis, cDNA cloning, and reverse transcription-PCR
O
Outcome
3'-terminal sequence of HCV genome RNA
Identifies a highly conserved 98-base sequence at the 3' terminus of the HCV genome, likely essential for viral replication.
Cite This Study
Kolykhalov et al. (1996) studied Hepatitis C virus. The hepatitis C virus genome RNA terminates with a highly conserved novel sequence of 98 bases, including a 46-base stable stem-loop structure, likely required for authentic HCV replication.