Secondary structure determination of the conserved 98-base sequence at the 3' terminus of hepatitis C virus genome RNA
Key result
Enzymatic and chemical approaches revealed a stable stem-loop structure within the 3'-terminal 46 bases of the HCV RNA genome, while the 5' 52 nucleotides appear less ordered.
Population
RNA transcribed in vitro from cloned cDNA of the hepatitis C virus (HCV) genome
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May guide HCV RNA-targeted antivirals; leaves open functional validation in cellular systems.
Structured PICO
P
Population
RNA transcribed in vitro from cloned cDNA of the hepatitis C virus (HCV) genome
E
Exposure
Enzymatic and chemical approaches to define secondary structure
O
Outcome
Secondary structure of the 3'-terminal 98-base element
The 3'-terminal 46 bases of the HCV genome RNA form a stable stem-loop structure, providing a basis for identifying interacting host or viral proteins.
Cite This Study
Blight et al. (1997) studied Hepatitis C virus (HCV). Enzymatic and chemical approaches was evaluated on Secondary structure of the 3'-terminal 98-base sequence. Enzymatic and chemical approaches revealed a stable stem-loop structure within the 3'-terminal 46 bases of the HCV RNA genome, while the 5' 52 nucleotides appear less ordered.