End labeling with T4 RNA ligase combined with gel sequencing provides a rapid and accurate method for determining the complete length spectrum of 3' terminal poly(A) tracts in RNA.
Variable poly(A) lengths in animal viral RNAs do not inform clinical practice; leaves open their functional roles in replication.
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.
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Ahlquist et al. (1979) studied this question.
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