High performance imaging and sensing systems for biomedical applications fabricated with 2-Photon Polymerization based additive manufacturing.

This presentation focuses on examples of devices and components to be used in actual biological and biomedical applications and manufactured in larger quantities. Specifically, we discuss additive manufacturing (full 3D and 2.5D grayscale modes) based on 2PP technology, which allows features of 100nm-150nm and surface roughness of 10-20 nm – sufficient for optical quality components. In addition, the printing volume allows parts of up to 50x50x22 mm3 and thus broader range of possible designs. Here we demonstrate two system examples: (1) high performance (NA=0.6, FOV=200microns, OD = 3.0 mm) hybrid endoscopic microscope objective for 2-photon imaging and diagnostics and (2) image mapping spectrometer for cell signaling in SPIM (Selective Plane Illumination Microscopy) configuration. In both cases we discuss performance of manufactured components and design strategy to optimize both printing time and component/system quality. Presented prototypes demonstrate high level of integration, compact dimensions and design flexibility. Results include high resolution imaging performance (miniature endo-microscopic objective) and snapshot spectral imaging capabilities in cell signaling.