What question did this study set out to answer?

The study aims to examine how maternal nutrition affects gene and mRNA expression in the skeletal muscle of beef cattle offspring.

February 8, 2026Open Access

Maternal nutrition alters mRNA isoform expression, usage, and splicing dynamics in skeletal muscle of beef cattle offspring

Key Points

The study aims to examine how maternal nutrition affects gene and mRNA expression in the skeletal muscle of beef cattle offspring.
Assigned 126 pregnant Nellore cows to three dietary treatments: NP, PP, and FP.
Collected muscle samples from offspring at 676 ± 28 days of age for RNA sequencing.
Analyzed differential gene expression and transcript usage using edgeR and IsoformSwitchAnalyzeR packages.
Conducted over-representation analysis with g:Profiler.
Identified 27,412 genes and 111,185 transcripts, with modest gene-level differences (16 genes) but stronger transcript-level effects.
FP × NP showed the greatest transcript usage changes with 14 DTEs and 87 DTUs.
Isoform switching in key genes like SLC7A8 and SORBS3 affected coding potential and muscle function.
Significant metabolic pathways related to amino acid metabolism and immune regulation were highlighted.

Abstract

Background Maternal nutrition during gestation plays a critical role in fetal muscle development and long-term metabolic programming; however, its persistent molecular effects on offspring skeletal muscle remain unclear. Therefore, the main objective of this study was to investigate the influence of prenatal nutrition on long-term differential gene expression (DGE), differential mRNA transcript expression (DTE), and differential transcript usage (DTU) in skeletal muscle of beef cattle. Methods A total of 126 pregnant Nellore cows were assigned to three dietary treatments: mineral supplementation only (NP), protein-energy supplementation during late gestation (PP), and protein-energy supplementation throughout gestation (FP). At 676 ± 28 days of age, muscle samples were collected from offspring for RNA sequencing. The DGE and DTE analyses were performed using the edgeR package, while DTU was evaluated with the IsoformSwitchAnalyzeR package. Over-representation analysis was conducted using g:Profiler. Results A total of 27,412 genes and 111,185 transcripts, including novel loci and isoforms were identified. Gene-level differences were modest (16 genes), whereas transcript-level analyses revealed stronger effects, with a higher number of significant expression and usage changes across conditions. The FP × NP comparison exhibited the greatest impact on gene expression, with 14 DTEs and 87 DTUs, compared with 12 and 30 in PP × NP, and 10 and three in FP × PP, respectively. Isoform switching was observed in key genes including SLC7A8 , SLC25A30 , SORBS3 , and CDH13 genes, influencing coding potential, functional domains, and mRNA stability, with potential consequences for amino acid transport, cytoskeletal organization, and muscle regeneration. Functional enrichment analyses highlighted significant metabolic pathways related to amino acid and biotin metabolism, intracellular trafficking, and immune regulation. Conclusion Overall, prenatal nutrition, particularly protein-energy supplementation throughout gestation in comparison to mineral supplementation, modulates offspring muscle mainly through transcript usage and splicing, suggesting long-term adaptive mechanisms beyond gene-level regulation.

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