Controlled CRISPR/Cas12a activation via DNAzyme-mediated splitting of chimeric substrate for lead detection

Key Points

• Lead detection increased by 3.5 times with CRISPR/Cas12a activation and DNAzyme technique.
• Efficient use of a chimeric substrate permits precise control in lead detection assays.
• Investigative approach employs DNAzyme splitting to activate CRISPR technology for improved outcomes.
• Highlights the potential for utilizing CRISPR/Cas12a in environmental monitoring and safety applications.