What question did this study set out to answer?

This study examines how Daifan Powder regulates the cGAS/STING signaling pathway to alleviate immune inflammation and improve bile duct injury in primary biliary cholangitis.

March 6, 2026

Mechanism ofitalic Daifan Powder /italic（黛矾散） regulating cGAS/STING signaling pathway to medicate immune inflammation to improve intrahepatic bile duct injury in primary biliary cholangitis

JXJUN XUInstitute of Plant Protection SGSheng GuoWuhan Textile University LZLihan ZhouHubei University of Chinese Medicine

Key Points

This study examines how Daifan Powder regulates the cGAS/STING signaling pathway to alleviate immune inflammation and improve bile duct injury in primary biliary cholangitis.
Mice were divided into control, model, and Daifan Powder treatment groups.
Treatment included various doses of Daifan Powder administered orally for 28 days after model induction.
Liver indices and biochemical markers were measured, including gamma-glutamyl transferase and alanine aminotransferase levels.
Histopathological changes in liver tissue were analyzed, and ELISA measured inflammatory cytokines.
mRNA levels of cGAS and STING were assessed by RT-PCR.
The model group showed increased liver index and elevated liver enzymes compared to the control group (P<0.01).
Inflammatory cells and collagen deposition were noted in liver tissues of the model group.
Treatment with Daifan Powder significantly improved liver parameters and reduced inflammation and collagen deposition (P<0.01).
cGAS, STING, and other signaling proteins were upregulated in the model group, but restored in treatment groups.

Abstract

目的探讨黛矾散调控环鸟苷酸-腺苷酸合成酶（cGAS）/干扰素基因刺激蛋白（STING）通路影响免疫炎症，改善原发性胆汁性胆管炎（PBC）肝内胆管损伤的机制。方法 C57BL/6小鼠分为对照组，模型组，熊去氧胆酸（100 mg kg-1）组，黛矾散低、中、高剂量（90，180，360 mg kg-1）组，造模后给药，连续灌胃28 d。测定小鼠肝脏指数；检测生化指标γ-GT、TBA、TBIL、AST、ALT含量；观察肝组织病理学改变；ELISA法检测TNF-α、IL-6、IL-1β、IFN-β、IL-17A含量；RT-PCR法检测cGAS、STING mRNA水平；WB法检测cGAS、STING、TANK结合激酶（TBK1）、p-TBK1、干扰素调节因子3（IRF3）、p-IRF3、NF-κB、p-NF-κB水平。结果与对照组比较，模型组肝指数升高（PPPP 结论黛矾散改善PBC小鼠肝内胆管损伤可能与cGAS/STING信号通路抑制免疫炎症相关。

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