Exploring the mechanism of antidiarrheal effect of modified italicRenshen Wumei Decoction/italic （人参乌梅汤） on diarrhea rats based on the SCFAs-GPR41/43 signaling pathway

Key Points

• To explore the effect of modified Renshen Wumei Decoction on the SCFAs-GPR41/43 signaling pathway and its antidiarrheal mechanism.
• Created diarrhea model in rats using senna leaf administration and dietary imbalance for 14 days.
• Rats were divided into model, western medicine, and traditional medicine groups for 7 days of treatment.
• Measured serum short-chain fatty acids (SCFAs) levels and inflammatory cytokines using ELISA.
• Assessed expression of targeted genes and proteins in colon tissue via RT-PCR and Western blot.
• SCFAs levels, including propionate and butyrate, significantly decreased in the model group compared to the control group.
• Both medicine groups significantly increased SCFAs levels compared to the model group, with traditional medicine showing greater increases.
• Gene and protein expressions of Occludin, Claudin-1, and GPR41/43 were reduced in the model group but restored by treatments.
• Inflammatory cytokines were altered in the model group, with treatments downregulating IL-1β and upregulating IL-10.