Localized catalytic hairpin assembly-based DNA tetrahedral sensor for sensitive and rapid miRNA detection in living cells
Key Points
The aim is to develop a fast and sensitive sensor for detecting miRNA using catalytic hairpin assembly.
Utilized catalytic hairpin assembly for signal amplification.
Designed a DNA tetrahedral structure for enhanced detection.
Conducted experiments in living cells to assess responsiveness.
Achieved rapid detection of miRNA in real-time.
Demonstrated high sensitivity to low-concentration targets.
Showed that the sensor works effectively in living cell environments.
Abstract
Catalytic hairpin assembly (CHA) signal amplification strategies have been employed for the detection of lowconcentration targets, but it requires several hours of reaction time, which poses a challenge for real-time...