Natural killer cells labeled with radionuclide-embedded double-layer gold nanoparticles conjugated to a mitochondrial localization sequence for PET/CT imaging and NK cell–based immunotherapy

Key Points

• This research aims to enhance PET/CT imaging techniques through the use of radionuclide-embedded gold nanoparticles labeled on natural killer cells.
• Conjugation of gold nanoparticles with a mitochondrial localization sequence
• Labeling of natural killer cells with radionuclide-embedded double-layer gold nanoparticles
• Conducting PET/CT imaging to monitor NK cell dynamics
• Stable labeling of NK cells was achieved
• High-sensitivity imaging techniques improved real-time monitoring capabilities
• The method supports preclinical validation for immunotherapy applications