Native Mass Spectrometry Reveals Binding Modes of the Tumor Suppressor Protein p53 to Different DNA Response Elements

Key Points

• This research focuses on understanding how the tumor suppressor protein p53 binds to different DNA response elements.
• Utilized native mass spectrometry to analyze binding interactions between p53 and DNA response elements.
• Investigated the formation of tetrameric complexes and dimer-dimer interactions.
• Identified that binding of a single dimer to an isolated half-site is insufficient for stable tetramer formation.
• Demonstrated the critical role of dimer-dimer interactions in stabilizing the tetrameric p53:DNA complex.