Structure-Guided Engineering of an Aromatic Dioxygenase from Coniochaeta pulveracea for Improved Catalysis of Lignin-Related Aromatic Olefins

Key Points

• To enhance the catalytic properties of an aromatic dioxygenase from Coniochaeta pulveracea for better processing of lignin-related compounds.
• Structure-guided engineering of aromatic dioxygenase
• Characterization of catalytic activity
• Assessment of coenzyme-independent properties
• Increased catalytic efficiency observed in engineered dioxygenase
• Enhanced oxidative cleavage of C=C bonds in aromatic olefins
• Production of valuable aldehydes and ketones improved