Horseradish Peroxidase: Analytical Capabilities in the Determination of Zearalenone

The possibility of using the horseradish peroxidase (HRP)–phenol enzyme–substrate system in the development of an amperometric immunoenzyme sensor for the determination of the mycotoxin zearalenone (ZEA) was shown. New immunoenzyme amperometric sensors based on printed graphite electrodes, including those modified with a fullerene/gold nanoparticles and HRP composite, were proposed for the determination of ZEA in a concentration range from 1 × 10–11 to 1 × 10–6 M, and cd was 5 × 10–12 M The binding constant of immune complexes (Ka = (5.3 ± 0.2) × 108 mol–1) and the percentage of cross reactions, which was <2.5% for patulin and <1.3% for deoxynivalenol, were estimated. A procedure for the determination of the mycotoxin ZEA in food products at an MPC level or below with RSD of no higher than 0.063 using the proposed immunoenzyme sensors was developed and tested.