PURPOSE: We investigated components of the immune tumor microenvironment as determinants of clinical outcome to anti-PD-1 therapy in patients with metastatic clear cell renal cell carcinoma (mccRCC) treated with frontline nivolumab in the phase 2, non-randomized HCRN GU16-260 trial. EXPERIMENTAL DESIGN: Pre-treatment primary ccRCCs from 72 patients were analyzed by multiplex immunofluorescence and image analysis to assess non-terminally exhausted CD8+ (CD8⁺PD-1⁺TIM3⁻LAG3⁻) tumor-infiltrating lymphocytes (TILs), PD-1+ regulatory T cells (Tregs), total and peritumoral tertiary lymphoid structures (TLS), and CD163+ tumor-associated macrophages (TAMs). Clinical endpoints included objective response rate (ORR) and progression-free survival (PFS). RESULTS: Densities of CD8⁺PD-1⁺TIM3⁻LAG3⁻ TILs, total and peritumoral TLS, and CD163⁺ TAMs, as continuous variables, were associated with improved ORR (Odds Ratio: 1.54 1.10-2.16 for TILs; 1.18 1.02-1.37 for total TLS; 1.10 1.02-1.18 for peritumoral TLS; 2.21 1.33-3.69 for TAMs) and longer PFS (Hazard Ratio: 0.79 0.67-0.95 for TILs; 0.92 0.85-0.99 for total TLS; 0.94 0.90-0.98 for peritumoral TLS; 0.77 0.61-0.97 for TAMs). Although % of PD-1+ Tregs as continuous variable was not associated with outcomes, at an optimal cut-off, high % of PD-1+ Tregs tended to be associated with lower ORR (12.5% vs 43.6%, p=0.093) and was associated with shorter PFS (3.4 vs 10.9 months, p<0.001). The biomarkers were not strongly correlated with each other and their integration in multi-biomarker models further stratified outcomes. CONCLUSION: Individual immune cell populations within the ccRCC microenvironment are associated with response/resistance to frontline anti-PD-1 therapy. Our findings support the development of combined immune marker models to identify patients with divergent outcomes.
Ahmar et al. (Mon,) studied this question.
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