Polymorphic variants of lncRNA genes LINC02227, LINC00305, and CDKN2B-AS1 were significantly associated with T2DM, and a risk model including these loci predicted T2DM with an AUC of 95.30%.
Case-Control (n=1,010)
Are polymorphic variants of lncRNA genes associated with the development of Type 2 Diabetes Mellitus?
Polymorphic variants of specific lncRNA genes are associated with T2DM and related metabolic traits, and a risk model including these loci, sex, age, and BMI can predict T2DM development with high sensitivity and specificity.
Effect estimate: OR 1.43
p-value: p=0.0017
Type 2 diabetes mellitus (T2DM) is a disease characterized by increased blood glucose, formed as a result of impaired mechanisms of insulin binding to cells. DNA samples of patients with T2DM (N = 535) and healthy individuals (N = 475) were used. We identified the association of lncRNA gene loci LINC02227 rs2149954 (OR = 0.76, P = 0.0083, PFDR = 0.017), LINC00305 rs2850711 (OR = 1.43, P = 0.0017, PFDR = 0.004), and CDKN2B-AS1 rs4977574 (OR = 0.70, P = 0.0001, PFDR = 0.0003) in an additive model with T2DM. The LINC00305 gene locus rs2850711 showed association with fasting glucose level (P = 0.023) and C-peptide level (P = 0.00001); LINC02227 rs2149954 and CDKN2B-AS1 rs4977574 loci showed association with hypertension. MALAT rs619586 gene polymorphism was associated with C-peptide (P = 0.017), LDL (P = 0.012), and total cholesterol levels (P = 0.01). CDKN2B-AS1 rs4977574 gene polymorphism showed association with C-peptide levels (P = 0.027). SNP LINC02227 rs2149954 was associated with obesity (P = 0.0011, PFDR = 0.008). MEG3 rs7158663 gene polymorphism was associated with post infarct cardiosclerosis (P = 0.02). ROC curve analysis showed that the studied loci and variables such as sex, age of subjects, and BMI level included in the construction of the risk calculation model could predict the development of T2DM with a sensitivity of 98.0% and specificity of 97.0%; the area under the curve (AUC) was 95.30% (95% CI 93.50–97.40).
Кочетова et al. (Sun,) conducted a case-control in Type 2 diabetes mellitus (n=1,010). lncRNA gene loci polymorphisms (LINC02227, LINC00305, CDKN2B-AS1) vs. Healthy individuals was evaluated on Association with T2DM (LINC00305 rs2850711) (OR 1.43, p=0.0017). Polymorphic variants of lncRNA genes LINC02227, LINC00305, and CDKN2B-AS1 were significantly associated with T2DM, and a risk model including these loci predicted T2DM with an AUC of 95.30%.