What does this research mean for the field?

Adapting existing poliovirus diagnostic RT-PCR assays to a real-time RT-PCR format using degenerate primers and probes containing deoxyinosine residues enables highly specific, rapid, and large-scale identification of poliovirus field isolates. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

April 23, 2009Open Access

Rapid Group-, Serotype-, and Vaccine Strain-Specific Identification of Poliovirus Isolates by Real-Time Reverse Transcription-PCR Using Degenerate Primers and Probes Containing Deoxyinosine Residues

DKDavid R. KilpatrickSouthern Illinois University Carbondale CYChen-Fu YangCenters for Disease Control and Prevention KCKaren ChingINTI International University

Structured PICO

Population

Poliovirus field isolates

Intervention

Real-time reverse transcription-PCR (rRT-PCR) using degenerate primers and probes containing deoxyinosine residues

Comparator

Previously described poliovirus diagnostic RT-PCR assays

Outcome

Rapid group-, serotype-, and vaccine strain-specific identification of poliovirus isolatessurrogate

The development of a real-time RT-PCR assay enables rapid and large-scale identification of poliovirus field isolates for the WHO Global Polio Laboratory Network.

Abstract

We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.

AI에게 질문

Bookmark

View Full Paper