The development of a real-time RT-PCR assay enables rapid and large-scale identification of poliovirus field isolates for the WHO Global Polio Laboratory Network.
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May accelerate poliovirus surveillance in labs; extends prior RT-PCR assays but requires field validation before network adoption.
We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.
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Kilpatrick et al. (2009) studied this question.
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