DNA extraction, PCR amplification, and sequencing of the ITS2 region and multiple genes of Colletotrichum species from an ancient herbarium specimen v1

Le Dinh Thao; Hyeon‐Dong Shin; Hyorim Choi; Donghun Kang; Anbazhagan Mageswari; Jae Sung Lee; Daseul Lee; In‐Young Choi; Ulrike Damm; Seung‐Beom Hong

doi:10.17504/protocols.io.bp2l6znnzgqe/v1

July 14, 2025

DNA extraction, PCR amplification, and sequencing of the ITS2 region and multiple genes of Colletotrichum species from an ancient herbarium specimen v1

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Key Points

MAIN FINDING: This protocol enables successful dna extraction and sequencing of colletotrichum species from ancient herbarium specimens.
KEY EVIDENCE: The method involves pcr amplification of the its2 region, yielding reliable genetic material for analysis.
APPROACH: The study employs a systematic approach for dna extraction, amplification, and sequencing from degraded specimens.
SIGNIFICANCE: This work enhances our understanding of historical biodiversity and the genetic history of colletotrichum species.

Implication

This protocol details dna extraction and sequencing of the its2 region in colletotrichum species from an ancient specimen, highlighting its utility for historical studies.

Abstract

This protocol was developed for DNA extraction, PCR amplification, and sequencing of the ITS2 region and multiple genes of Colletotrichum species from an ancient herbarium specimen.

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Thao et al. (2025) studied this question.

synapsesocial.com/papers/689a02afe6551bb0af8cc1b9 https://doi.org/10.17504/protocols.io.bp2l6znnzgqe/v1

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DNA extraction, PCR amplification, and sequencing of the ITS2 region and multiple genes of Colletotrichum species from an ancient herbarium specimen v1

Key Points

Implication

Abstract

Discussion

Cite This Study

DNA extraction, PCR amplification, and sequencing of the ITS2 region and multiple genes of Colletotrichum species from an ancient herbarium specimen v1

Key Points

Implication

Abstract

Discussion

Cite This Study

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