Vitamin B12 Absorption in a Community is ‘Continuously’ Distributed and Influences Response to Long-term Oral Supplementation

Background Insufficient dietary intake and/or reduced gastrointestinal absorption lead to low vitamin B 12 (B-12) status. In a secondary data analysis of the results of the B-12 absorption (CobaSorb) test and B-12 supplementation trial in a B-12 insufficient Indian rural community, we explored characteristics of B-12 absorption and its association with post-supplementation plasma B-12 concentration. Objective To study i) the distribution of plasma holo-transcobalamin (holoTC) response during the CobaSorb test, and ii) determinants of plasma B-12 response to long-term, oral supplementation with ‘physiological dose’ B-12. Methods The participants (parents and children in the Pune Maternal Nutrition Study) first underwent a B-12 absorption study Subsequently, they participated in a 12-month-long, double-blind RCT of daily oral B-12 (0, 2, or 10 µg) and folic acid (0 or 200 µg). Circulating B-12 was measured at baseline and after 4 and 12 months of supplementation. A linear mixed-effect model was used to study the predictors of B-12 absorption and response to supplementation. Results Three hundred and thirteen participants included children (n=109, 57 boys, mean age 9y, weight 21.9 kg, BMI 13.6 kg/m 2 ), mothers (n=108, 30y, 47.7 kg, 19.3 kg/m 2 ), and fathers (n=96, 37y, 59.3 kg, 21.4 kg/m 2 ). The plasma holoTC response during the absorption test was continuously distributed and was negatively associated with weight and positively with the dose of B-12. Response to long-term B-12 supplementation was positively predicted by absorption test response, dose of B-12, length of supplementation, and compliance. Conclusions The continuous distribution of plasma holoTC response during the absorption test supports a graded absorption of B-12. CobaSorb protocol could be used to unravel the intricacies of B-12 absorption. Clinical Trial Registry number and website where it was obtained ISRCTN59289820. DOI: https://doi.org/10.1186/ISRCTN59289820 https://www.isrctn.com/