Development and validation of analysis method for sarmentosin in Sedum sarmentosum extract

Sedum sarmentosum (SS) is a medicinal plant with a range of physiological benefits. To develop functional food ingredients and products, functionality, and safety must be proven through the standardization of raw materials. In this study, we established sarmentosin as a marker compound for SS and aimed to develop an analysis method for its quantification in SS extracts. As a result of validating the developed sarmentosin analysis method, the calibration curve showed linearity with a correlation coefficient (R2) value of 0.999. The precision and accuracy of the analysis method were confirmed for intra-day and inter-day. As a result, the intra-day precision was found to be 1.18-1.42% and inter-day precision to be 0.51-1.11%. Also, intra-day accuracy was found to be 99.34-101.29% and the inter-day accuracy to be 99.54-103.23%. Furthermore, the detection limit of sarmentosin was 0.27 μg/mL, and the quantification limit was 0.81 μg/mL. We conducted a study to optimize extraction conditions using a proven sarmentosin analysis method, and confirmed that water extraction at 90°C for 8 h was the optimal condition (sarmentosin content 6.53±0.17 mg/g). The analysis method established in this study provides a reliable tool for the quality control of SS, enhancing its potential utility.