Genomic Resources for Fusarium sambucinum, a Causal Agent of Fusarium Canker of Hop and Dry Rot of Potato

Fusarium sambucinum is a plant pathogen involved in multiple pathosystems including dry rot of potato (Solanum tuberosum) and Fusarium canker of hop (Humulus lupulus). As a causal agent of Fusarium canker, the pathogen is becoming increasingly important in the Pacific Northwest due to rising disease incidence over the last decade. To better understand the pathogen and facilitate future genomics research, two F. sambucinum isolates originally obtained from two common hosts in the United States, hop and potato, were sequenced and assembled using long read sequencing technology. Four chromosomes were identified along with a mitochondrial genome sequence, resulting in a total genome size of 38 Mb. The hop-derived genome assembly, OSU_Fsam_1.1, is the first F. sambucinum genome associated with Fusarium canker of hop and is of high quality with 89× coverage, a BUSCO completeness of 99.7%, and an N 50 of 9.53 Mb. A total of 13,388 genes were annotated, including 39 secondary metabolite biosynthetic gene clusters, 1,219 carbohydrate-active enzymes, and 474 candidate effectors. The potato-derived genome, OSU_Fsam_2.1, has 185× coverage, 97.3% completeness, and N 50 of 9.65 Mb. OSU_Fsam_1.1 and OSU_Fsam_2.1 are the first publically available chromosome-level assemblies for F. sambucinum and the first assembled mitochondrial genome of F. sambucinum. These resources will greatly enhance future genomics research in the Fusarium sambucinum species complex.