Mechanistic elucidation of enzymatic C-glycosylation: facilitation by proton transfer to UDP-glucose

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This is a In Vitro Study study.

Daisuke Terada; Taichi Inagaki; Miho Hatanaka

doi:10.1039/d5ra02643a

January 1, 2025RSC AdvancesOpen Access

Mechanistic elucidation of enzymatic C-glycosylation: facilitation by proton transfer to UDP-glucose

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Key Points

UDP-glucose plays a dual role in C-glycosylation—acting as a glucose donor and a proton acceptor, facilitating the reaction.
DFT calculations demonstrate the intricate mechanism of proton transfer, underpinning enzymatic processes essential for glycosylation.
This study highlights the importance of recognizing UDP-glucose in enzymatic functions, deepening our understanding of carbohydrate chemistry.
Insights gained could inform future enzymatic studies and applications in areas like drug development and synthetic biology.

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Implication

DFT calculations reveal UDP-glucose as both glucose donor and proton acceptor in C-glycosylation, suggesting new insights.

Abstract

DFT calculations revealed a dual role of UDP-glucose in C -glycosylation: not only as a glucose donor but also as a base that accepts a proton from the substrate, thereby initiating the reaction.

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Cite This Study

Terada et al. (2025) studied this question.

synapsesocial.com/papers/68a36ddf0a429f7973331164 https://doi.org/10.1039/d5ra02643a

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Mechanistic elucidation of enzymatic C-glycosylation: facilitation by proton transfer to UDP-glucose

Key Points

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Implication

Abstract

Discussion

Cite This Study

Mechanistic elucidation of enzymatic C-glycosylation: facilitation by proton transfer to UDP-glucose

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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