Eco-friendly Electrochemical Sensor for Quantification of Bumadizone in Pure, Pharmaceutical Dosage Form and in Spiked Human Plasma Using Pencil Graphite Electrode

Abstract Bumadizone calcium hemihydrate is a non-steroidal anti-inflammatory drug that is used to treat rheumatoid arthritis and post-traumatic edema through blocking prostaglandin synthesis. This work presents, for the first time, an electrochemical method has been developed for determination of bumadizone calcium hemihydrate in bulk, pharmaceutical dosage form and in spiked human plasma based on the oxidation nature of bumadiazone using pencil graphite electrode as a working electrode. The electrochemical oxidation of bumadizone at pencil graphite electrode surface in phosphate buffer pH 6.4 was investigated using cyclic and differential pulse voltammetry between -0.1 and 1.3 V against Ag/AgCl reference electrode. Experimental parameters of the suggested method as pH, scan rate, step potential, pulse amplitude and pulse width (modulation time) were well investigated and optimized. The proposed differential pulse voltametric method was found to be linear over the range 5.0×10⁻⁷to 5.0×10⁻⁵M with high correlation (r= 0.999). The limit of detection (LOD) and limit of quantification (LOQ) were estimated to be 1.6×10⁻⁷ M, and 5.3×10⁻⁷M, respectively. Using pencil graphite electrode offers extra advantages to the proposed method of being widely commercially available, cost-effective, simple, disposable and mechanically rigid electrodes. The method was found to be efficiently applicable in spiked human plasma permitting bioequivalent or pharmacokinetic study in real human plasma samples. Greenness of the proposed method was evaluated using Eco-scale scoring tool revealing excellent greenness of the analytical method.