Isolation of Toxoplasma gondii from Placental Tissue and its Effect on Malondialdehyde Levels in Pregnant Women in Al-Anbar Province of Iraq

Background: Toxoplasmosis is recognized as among the prevalent ailments globally affecting mammalian creatures. Around a third of the Earth's inhabitants are afflicted with the condition. Miscarriage, also known as spontaneous abortion or pregnancy loss, pertains to an unviable intrauterine pregnancy that occurs within 20 weeks of gestation. Early pregnancy loss, which occurs throughout the first trimester, is the most common miscarriage type.Methods: Alternative techniques that do not involve enzymes have been employed to separate the Toxoplasma gondii parasite from placentas that have been aborted or not in women with toxoplasmosis in Al-Anbar Province. The purpose of such techniques is to preserve the parasite's viability while it rapidly multiplies inside placental tissue. Moreover, the current investigation measures the amounts of malondialdehyde (MDA) to determine how the parasite affects lipid peroxidation.Results: Upon examining the pellet and suspension obtained from the placental solution, the microscopic analysis revealed the existence of bradyzoite in a majority of the identified specimens, along with the appearance of the swiftly reproducing tachyzoite phase in some. Lipid Peroxidation measurements showed that the infected samples' MAD levels had significantly increased relative to control samples.Conclusion: Non-enzymatic methods of isolating the toxoplasma parasite are better at maintaining the vitality of the different phases of the parasite. Moreover, the parasite's effect is evident in raising lipid peroxidation levels and increasing risk factors.Keywords: Toxoplasma gondii; Malondialdehyde MDA; Lipid Peroxidation; AbortionEditorial Expression of Concern20 June 2025: Following publication of this paper, the internal audit (consequent to concerns on quality raised by Web of Science) notified Advancements in Life Sciences about problems in use of English language. By this Editorial Expression of Concern, we alert the scientific community as we address the errors.Editorial Note26 June 2025: You are viewing the latest version of this article having minor corrections related to the use of English language. Expression of concern is hereby revoked.