Fibroblast culture from rodent/lagomorph ear v1

Ashley W. Seifert; Ebenezer Aryee; Ajoy Aloysius

doi:10.17504/protocols.io.yxmvmb3kog3p/v1

August 14, 2025Open Access

Fibroblast culture from rodent/lagomorph ear v1

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Key Points

Culturing fibroblasts from ear pinnae and fetal tissue is effective for various experimental uses, and ensuring IACUC approval is crucial for ethical compliance.
Primary fibroblasts can be isolated from rodents like Mus and Rattus, and from lagomorphs such as Oryctolagus, providing versatile biological samples.
This protocol describes methods for handling delicate tissues, emphasizing sterility and specific conditions to support fibroblast growth.
Proper techniques in fibroblast culture could lead to improved experimental outcomes in mammalian research, indicating the need for careful attention to procedures.

Implication

This protocol outlines the process for culturing fibroblasts from rodents and lagomorphs, highlighting necessary IACUC approvals.

Abstract

This protocol describes the procedure for isolating and culturing either primary fibroblasts from the ear pinnae or fetal fibroblasts from the ear buds and head skin of rodents and lagomorphs, including Acomys, Mus, Rattus, and Oryctolagus, used in the Seifert Lab. Note that any protocol involving animals should be approved by your Institutional Animal Careand Use Committee (IACUC) before use.

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Cite This Study

Seifert et al. (2025) studied this question.

synapsesocial.com/papers/68af4cd8ad7bf08b1ead6334 https://doi.org/10.17504/protocols.io.yxmvmb3kog3p/v1

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Fibroblast culture from rodent/lagomorph ear v1

Key Points

Implication

Abstract

Discussion

Cite This Study

Fibroblast culture from rodent/lagomorph ear v1

Key Points

Implication

Abstract

Discussion

Cite This Study

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