Comprehensive Evaluation of the Safety and Efficacy of MOSY PHYCOLLREPAIR: A Novel Fermented Black Bee Honey‐Derived Cosmetic Ingredient

Background MOSY PHYCOLLREPAIR is an innovative cosmetic ingredient extracted from fermented blank bee honey, specifically engineered for dermatological applications. Objective This study seeks to thoroughly evaluate the safety and diverse efficacy profiles of MOSY PHYCOLLREPAIR within cosmetic formulations. Methods The safety of MOSY PHYCOLLREPAIR was determined through zebrafish embryo developmental toxicity assays. Its dermatological benefits were assessed via a comprehensive series of in vivo zebrafish embryo tests, encompassing antioxidation, anti‐inflammation, modulation of type I collagen, elastin, and sirt1 gene expression, wound healing, skin barrier integrity, UV protection, and skin whitening. Results MOSY PHYCOLLREPAIR demonstrated non‐toxicity to zebrafish embryos at concentrations up to 3.08 mg/mL. It showed significant efficacy across multiple parameters: 29.10%–62.48% anti‐ROS activity, 11.08%–27.84% anti‐inflammatory effect, 60.25%–295.67% ATP‐enhancing efficacy, and 6.52%–17.80% melanin inhibition at concentrations ranging from 25 to 500 μg/mL. It also upregulated the expression of type I collagen genes col1a1 by 87.67% to 281.61%, col1a2 by 54.08% to 165.14%, astin gene elna by 55.81% to 174.03%, and sirt1 gene by 60.25%–295.67%. Additionally, MOSY PHYCOLLREPAIR enhanced wound healing by 8.33%–19.44% at concentrations between 5 and 100 μg/mL, provided 39.36% to 70.26% skin barrier protection against chemical peel at 100 to 500 μg/mL, and offered 20.15%–68.45% UVB irradiation protection at 200 to 2000 μg/mL. Conclusion This study marks the first application of fermented blank bee honey as an active cosmetic ingredient. The findings conclusively establish MOSY PHYCOLLREPAIR as a safe and highly effective cosmetic component, delivering a broad range of anti‐aging and skin health benefits.