Effect of Helium Neon Laser Irradiation on Calcium Levels in Blood "An Experimental Study"

This study was conducted to find out the physiological effect of low-level Helium-Neon radiation on serum calcium in human blood. The laser was of 5 mW power, wavelength being 632.8 nm, with a spot size of about 0.03 cm² to ensure uniform exposure. Energy density of 1.0 J/cm² was delivered to the samples. Twenty healthy, nonsmoking volunteers of different age groups from 19 to 65 participated. Blood was drawn, serum was separated by centrifuging at 3000 RPM for about 3 to 5 minutes at 25 °C, and then the serum was divided into four portions: one portion being the control and the three other aliquots putting to laser exposure for 1, 3, and 5 minutes. In results, it was seen that after 1 minute of exposure, there were trivial changes in calcium levels (about 0.2-0.3 mg/dl average increase). Increasing exposure to 3 minutes seemed to noticeably raise variabilities in calcium concentrations (about 0.5-1.5 mg/dl average increase), indicating possible effects on the cell membrane permeability and enzymatic activity. With 5-minute exposure, the results diverged; some continued rising (say, sample 18: from 8.7 to 18.4 mg/dl, and sample 12: from 9.3 to 15.3 mg/dl), but others either stabilized or decreased just slightly. gender-dependent variations were observed: males showed more consistent increases in calcium levels with longer exposure times, whereas females exhibited more varied responses, with some samples showing significant elevations. This significant and differential increase in calcium levels is mainly attributed to the photobiomodulatory effect of the low-level helium-neon laser, which stimulates cellular processes without significant thermal effects.