Anatomical attributes of Nidularium minutum (Bromeliaceae) cultivated in vitro: implications for water conservation

Some anatomical attributes of Bromeliaceae are associated with water conservation and retention in plants under water-restricted conditions. These characteristics may facilitate the successful acclimatization of many species of this family following in vitro culture. This study aimed to characterize the anatomical structures of the vegetative organs of juvenile Nidularium minutum plants grown in vitro and to identify the attributes that could contribute to the successful ex-vitro establishment of this ornamental species. The anatomy of the vegetative organs of plants cultivated in vitro for 90 days was analyzed using optical and scanning electron microscopy. We observed structures such as leaf scales, leaf aquiferous parenchyma, and intracortical roots, which may help prevent dehydration, as well as colleters at the shoot apex that protect developing organs-features not commonly reported in Bromeliaceae. We conclude that this set of anatomical features could enhance the ex vitro establishment of N. minutum.