Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
August 15, 2025Open Access

Evaluation of Parallel Accumulation-Serial Fragmentation methods for metaproteomics using a model microbiome

View Full Paper
Ask AI
Bookmark
Share

Authors

RSRuben ShreshtaARAndrew T. RajczewskiKDKatherine Do

Discussion

Loading...

Member takes

Overview

This analysis finds diaPASEF method improves peptide and protein identification in a microbiome model, suggesting better quantification.

Key Points

  • diaPASEF identified 168% more peptide precursors compared to ddaPASEF, showcasing significant improvements in depth of coverage.
  • Quantitative measurements with diaPASEF demonstrated better precision, as 26 organisms had coefficients of variation below 20%, indicating reliability.
  • Assessment of methods involved a mock community with 28 species and 30 strains, spanning all three domains of life and bacteriophages.
  • Findings emphasize diaPASEF's potential in clinical and environmental studies for deeper functional characterization of microbiomes.

Cite This Study

Shreshta et al. (2025) studied this question.

synapsesocial.com/papers/68af55dead7bf08b1eadca10https://doi.org/10.1101/2025.08.13.670166
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Systematic evaluation of PASEF acquisition strategies in complex metaproteomes2026
  2. 2Data-Independent Acquisition Mass Spectrometry as a Tool for Metaproteomics: Interlaboratory Comparison Using a Model Microbiome2024 · 6 citations
  3. 3Assessing fecal metaproteomics workflow and small protein recovery using DDA and DIA PASEF mass spectrometry2024 · 7 citations
  4. 4Assessing fecal metaproteomics workflow and small protein recovery using DDA and DIA PASEF mass spectrometry2024
  5. 5Evaluation of Protein Identification and Quantification by the diaPASEF Method on timsTOF SCP2024 · 12 citations