Light‐Mediated Insulin and Amylin Secretion: Influence of Irradiation Parameters in Optogenetically Modified Mouse‐Pancreatic β Cells

Optogenetics offers a promising avenue for controlled insulin secretion in diabetes management using genetically modified cells. However, the influence of irradiation parameters on the co‐secreted hormone amylin remains largely unexplored. This study examined how blue light power densities (50, 100, and 200 mW/cm 2 ) under continuous and intermittent exposure affect hormonal secretion in channelrhodopsin‐2 expressing mouse β cells. Results showed that 10 s of continuous exposure at 50 mW/cm 2 increased insulin and amylin secretion by ~25% and 40%, respectively. Introducing intermittent dark intervals further enhanced secretion by ~35% for insulin and 70% for amylin at 50 and 100 mW/cm 2 respectively, correlating with intracellular calcium changes. Notably, light modulated the amylin‐to‐insulin molar ratio, peaking with a 34% increase during 5 s ‘on’‐10s ‘off’ exposure at 100 mW/cm 2 . This study provides the first evidence that light irradiation parameters can differentially influence hormonal secretion from engineered cells, offering a potential for tailored cell‐based diabetes therapies.