What type of study is this?

This is a In Vitro Study study.

August 25, 2025Open Access

Gadobutrol at High Concentrations Compromises Cell Viability and Genome Integrity: A Call for Prudent Use in Clinical Imaging

Key Points

High concentrations of gadobutrol significantly compromise cell viability and genome integrity, threatening safe clinical use.
Cell viability notably decreased at gadobutrol concentrations ranging from 10-100 mM, with statistical significance (p<0.0001).
Assessment utilized a CCK-8 kit for cytotoxicity and the Comet assay for examining genotoxicity effects in cells.
Findings underscore the need for careful consideration of gadobutrol use in clinical imaging protocols due to potential risks.

Abstract

Background: Gadobutrol is a paramagnetic and nonionic contrast agent developed as a gado-linium derivative. This agent is routinely used for diagnostic purposes. It is also preferred in resonance angiography and magnetic resonance imaging (MR) because it increases tissue contrast. The aim of this study was to determine the in vitro cytotoxic and genotoxic effects of gadobutrol on NIH3T3 cell line. Materials and Methods: NIH3T3 cell line was grown under standard conditions (37°C, 5% CO₂). CCK-8 kit was used to determine the cytotoxic effect. Genotoxicity potential was deter-mined by Comet assay. Results: Gadobutrol had no significant effect on cell viability at low concentrations, but as the concentration increased (10-100 mM), cell viability decreased statistically significantly (p0.0001). Conclusions: Gadobutrol showed marked cytotoxic and genotoxic effects in NIH3T3 cells at high concentrations.

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Cite This Study

Hüsunet et al. (Mon,) studied this question.

synapsesocial.com/papers/68af5d63ad7bf08b1eae085e https://doi.org/https://doi.org/10.35440/hutfd.1678465

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