Comparative Study of Taurine Extenders on the Cryosurvivability and Fertility in Buffalo Bull Semen.

The objective of this investigation was to explore the cryosurvivability and in-vivo fertility in buffalo bull semen extended in Tris taurine, Tris taurine BHT and Tris taurine CLC. The basic extender was Tris-citric acid- fructose egg yolk glycerol (TCFYG). Tris extender with zero taurine, zero BHT and zero CLC was kept as a control. The other extenders were Tris containing taurine (50Mm/l), Tris containing taurine and Butylated Hydroxytoluene BHT (1Mm BHT+60 Mm/L taurine) and Tris containing Taurine and cholesterol-loaded cyclodextrin CLC(1mg/ml CLC+60 Mm/L taurine). We exposed the extended semen to the freezing protocol. Taurine supplement to the preservation diluent ameliorated sperm quality as indicated by post freeze-thaw spermatozoal motility, normal acrosome , viability index and significant decrease of percent of premature capacitation. Sperm motility, viability index and sperm cell membrane fluidity (HOST) were obviously enhanced accompanied by the lowest significant percent of premature capacitation upon on adding taurine BHT in the freezing diluent. Significant decrease in post-thawing sperm motility with the advance of time in all the extenders was observed. Non-significant elevation of the total antioxidants (TAC) in TrisTaurine BHT and non-significant increase of malondialdehyde (MDA) in TrisTaurine CLC relative to the control were recorded. The superior conception rate (CR) was recorded in Tris Taurine BHT followed by Tris Taurine and Tris Taurine CLC if compared to the control. It could be concluded that, TrisTaurine BHT is considered the best ameliorating for sperm cryosurvivability and fertility followed by Tris Taurine and Tris Taurine CLC.