Intravaginal Co-delivery of Myoinositol and Metformin via Dual MPPs-Loaded Gel for PCOS Amelioration: An In-vivo Study in a Rat Model

Polycystic ovarian syndrome is a highly prevalent, multifaceted endocrinopathy among reproductive women. Current therapies have limited therapeutic success and compliance owing to multiple complications. Here, we developed MPPs-Gel formulation for efficient targeted intravaginal delivery that addresses the complementary mechanism of insulin resistance and other associated disorders. The MPPs size was observed to be 194.1 ± 3.54nm with the zeta potential of -6.45mV, depicting the acceptable value providing enhanced penetration across the vaginal delivery. The study investigated the effects of the MPPs-Gel formulation on insulin resistance and reproductive health in a PCOS rat model. The estrous cycle of rats revealed that the MPPs-Gel efficiently regulated, as validated by the presence of all the phases. Ultrasonographic study, the MPPs-Gel group demonstrated an approximately 50% reduction in ovarian size (4.8 ± 0.78mm) in comparison to the PC group (9.2 ± 0.84mm). Results demonstrated significant improvement in insulin sensitivity, with a notable recovery observed in the MPPs-Gel group. The findings revealed a marked reduction in serum androgen concentrations, alongside notable improvements in other endocrine hormones with enhanced regulation of the reproductive cycle and oocyte quality. Thus, our findings validated that MPPs-Gel could be a promising holistic treatment approach for the associated disorders in PCOS.