Evaluation of aza-BODIPY fluorophores in detecting biothiols demonstrates high sensitivity, indicating novel sensing mechanisms.
Two alternate aza-BODIPY fluorophores bearing a formyl-functional group were synthesized and evaluated for biothiol sensing. Both provide high binding constants (up to 9800 M-1) and low limits of detection (as low as 1 μM). More importantly, this study introduces an unconventional "turn-on" sensing mechanism, in which fluorescence enhancement arises from analyte-induced restriction of intramolecular rotation rather than analyte-triggered cleavage of quencher. This alternative strategy provides a promising platform for the development of next-generation fluorescent probes.
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Sadıkoğulları et al. (2025) studied this question.
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