Optimised mass spectrometry workflow identifies long-chain S-acylated peptides in HEK293T cells, suggesting new insights into lipid modifications.
Key Points
Long-chain S-acylated peptides can be effectively detected using an optimised mass spectrometry workflow, revealing important lipid modifications.
The study established that stability of long-chain S-acylated peptides is maintained under pH variations, but extended trypsin digestion reduces signal.
Fragmentation methods such as HCD were found to provide the most effective sequence information for long-chain S-acylated peptides compared to other methods.
Employing the new approach, dual acylation states of GNA13 and both long-chain S-acylation and prenylation of RhoB were successfully identified.