Estimation and analysis of the impact of habitats and chromosomes on trichome variation in Lilium amabile Palibian

The leaf trichome, a unique and distinctive feature of Lilium amabile Palibian, sets this species apart from the 15 endemic lilies grown in Korea. This experiment examined the leaf trichome type and its variation in density, length, and width by geographical region (habitat). It focuses on investigation how chromosome/b chromosome and trichome variation parameters (density, length, and width) are related in L. amabile Palibian. Altogether, 51 leaf samples were examined, each representing every accession from six geographical regions in Korea. The mature intact leaves of each population was preserved in an acetic/ethanol (1:3) solution for up to 14 days. Subsequently, leaf trichome observation was conducted using light microscopy, a reliable and widely accepted method in the field of such types of research. Trichomes, found on both sides of the leaf (adaxial and abaxial), varied in density, length, and width and were categorized into four groups i.e. conical, cylindrical, glandular, and hooked. The conical type of trichome was commonly recorded in every population and sample. The results suggested that the primary ecological factors of habitat could influence the shape, size, and density of leaf trichomes. The findings confirmed that ecology, elevation, sunlight, and chromosomal changes affect trichome morphology and density. It was also observed that chromosome/B chromosome changes do not significantly affect trichome type and density.