Supplementation of Phytase and Protease Enzymes on the Performance of KUB Chicken using the MSTN Gene Expression

Background: The KUB chicken industry faces several problems in early growth and active biological activity. One thing that needs to be done is the addition of phytase and protease enzymes to livestock feed. One effort to increase other livestock production is through molecular or genetic markers. The purpose of this study was to determine the effect of adding phytase and protease enzymes to the ration on the performance of KUB chickens, as well as to determine the expression of the MSTN gene on KUB chicken carcass production. Methods: A total of 35 blood samples were taken from superior Balitnak (KUB) native chickens, consisting of 8 blood samples from groups of male chickens with the same variety, age with feed added with phytase enzyme, samples were taken from male chickens with the same body weight given feed with a mixture of protease enzymes. The following methods were used to calculate body weight increase, feed intake and feed conversion ratio (FCR). Amplification of the myostatin gene (MSTN) in KUB chickens was carried out using the Polymerase Chain Reaction (PCR) technique. Result: Phytase and protease enzyme supplementation did not show a significant effect (P greater than 0.05) on the relative weight of chicken internal organs, but has the potential to increase digestive efficiency and metabolic function. Enzyme activity in the digestive tract is considered an important factor that can affect gut health and nutrient digestibility. MSTN gene transcript expression was detected in all enzyme doses investigated in this study. Myostatin (MSTN) gene expression in KUB chickens was associated with muscle development, growth rate, organ composition and body weight.