CO₂ Sequestration Potential and Soil Improvement Effects by Carbon‐Fixing Bacteria Isolated From Degraded Soils in Shendong Coal Mining Area Located in Northwest China

Efficient carbon‐sequestering microorganisms are crucial for enhancing soil quality and reducing carbon emissions, particularly in semiarid coal mining areas. In this study, we analyzed the soil carbon‐fixing microbial taxa and pathways in the Shendong mining area via high‐throughput sequencing, and concluded that their main carbon sequestering pathway is the reduced citrate cycle (rTCA cycle), which has the potential for microbial carbon sequestration. Bacterial strains with high CO 2 sequestration efficiency were isolated and identified, and their potential for improving soil carbon storage and quality was assessed. Through a systematic process of isolation, identification, and functional assessment, three dominant strains, Streptomyces marokkonensis FC1, Streptomyces viridochromogenes FC2, and Dyadobacter endophyticus FC3 with significant CO 2 sequestration capacity were obtained. Their adaptability and carbon fixation efficiency were confirmed through physiological, biochemical, and genetic analyses. The results from indoor 13 C isotope labeling experiments and 42‐day soil improvement experiments demonstrated that CO 2 sequestration and soil improvement effects were achieved in coal mine soils under semiarid conditions. Among the strains, FC2 presented the highest carbon fixation potential and soil improvement efficiency at approximately 8% soil water content. This highlights the potential application of carbon‐fixing bacteria in microbial‐based strategies for the ecological restoration of degraded mining soils.