Validated RP-H-P-L-C Method for the Simultaneous Determination of Betamethasone and Ofloxacin in Bulk and Pharmaceutical Formulations

The immobile fluid utilized for separation is a Kromosil® C18 H-P-L-C Column with a size of particle which is 5 μm & a pore size of 100 Å, measuring 250 mm in distance end to end & 4.6 mm in diameter (internal). The MP consists of KH2PO4 & Acetonitrile in a 55:45 ratio, maintained at a Flow Rate (FR) of 1.0 ml/min, with a maximum wave vector of 278 nm & a temp set at 30.0°C. The average retention times recorded for Betamethasone & Ofloxacin were 2.331 mins & 3.312 mins respectively. The percentage recovery was recorded at 100.09% for Betamethasone & 100.62% for Ofloxacin.