Optimization of Extraction Process for Kaempferia Parviflora in Vietnam Using Response Surface Methodology

Objective Kaempferia parviflora collected in the Bay Nui region of An Giang, Vietnam. This project aims to build an extraction process with optimized parameters using the RSM model to improve the quality and polyphenol and flavonoid content of extracts from KP. Method Kaempferia parviflora was extracted using the ultrasound method, with factors such as temperature, time, and solvent volume affecting the process. The biological activities were assessed using several methods, including the DPPH assay, ABTS assay, agar disk-diffusion method, and the Minimum Inhibitory Concentration (MIC) test. Results The content of polyphenols and flavonoids was evaluated to base on the RSM with the optimal parameters from the model (at 96 min, volume 15.4 mL, temperature 66 o C) of 81.57 (mgGAE).(gDW) −1 and 50.70 (mgQE).(gDW) −1 (R 2 = 0.999 and 0.993 > 0.75). The antioxidant capacity of the extract was found to be high with DPPH and ABTS results of 6.990 and 0.014 mg.mL −1 . Besides, the antibacterial ability of the sample was also recorded at the minimum inhibitory concentration of Escherichia coli of 10 mg.mL −1 and Staphylococcus aureus of 15 mg.mL −1 . Conclusions The study showed that the ultrasonic extraction method has shown superior time-saving ability. The extraction conditions were optimized to ensure high flavonoid and polyphenol content, with good activity. It can be seen that Kaempferia parviflora extract had great potential for application in the medical field.