New genome assembly reveals 40 chromosomes in Alectoris rufa, highlighting genetic diversity and adaptations.
<ns3:p>The red-legged partridge (<ns3:italic>Alectoris rufa</ns3:italic>) is a socio-economically important game bird in southern Europe. Despite previous efforts, achieving a high-quality, chromosome-level genome assembly has remained challenging. Here, we present a <ns3:italic>de novo</ns3:italic> phased, gapless reference genome of <ns3:italic>A. rufa</ns3:italic>, combining Nanopore long-read sequencing and Hi-C data from both sexes. The assembly resolves 40 nuclear chromosomes (38 autosomes and the 2 sex chromosomes, Z and W) and the mitochondrial genome, achieving chromosome-scale resolution and 99.1% completeness based on the Aves BUSCO dataset. This high-quality genome provides a critical resource for studying genetic diversity, sex-linked traits, and evolutionary adaptations.</ns3:p>
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