Observational analysis reveals miR-243 directs pUGylation of specific mRNAs in C. elegans, suggesting roles in gene regulation.
C. elegans RDE-3 adds poly UG tails to mRNAs targeted for silencing by the dsRNA-initiated RNA interference (RNAi) pathway. RDE-3 can also add p(UG) tails to some endogenous cellular mRNAs. Mechanisms directing RDE-3 to pUGylate specific mRNAs are not understood. Here we show that the miRNA miR-243 directs pUGylation of an intestine-specific mRNA y47h10a.5 , which leads to silencing of the mRNA. The data show that genome-encoded small regulatory RNAs are one mechanism by which RDE-3 can be directed to pUGylate specific mRNAs for gene regulation.
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Lowe et al. (2025) studied this question.
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