Protocol outlines purification of mRNA, shRNA, and sgRNA from in vitro transcription, highlighting yield and purity.
This is a protocol for purification of in vitro transcribed RNA via the Zymo Research Quick-RNA Miniprep Kit. It works with capped and uncapped mRNA (usually >600 nt) with or without poly(A)-tails , shRNA (49 nt) and single guide sgRNA (100 nt) and yields RNA of high purity and concentration.
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Christian CR Renicke (2025) studied this question.
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