Micropropagation and Acclimatization of Hypericum aucheri

Hypericum aucheri is a Hypericum species distributed in Türkiye as well as Greece and Bulgaria with notable pharmacological potential. Its medicinal importance underlines the need for effective propagation strategies. This study developed an in vitro micropropagation protocol using nodal explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins—6-benzylaminopurine (BAP) (0, 1, 2 mg L−1) or kinetin (KIN) (0, 1, 2 mg L−1)—combined with 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1 mg L−1) for shoot induction. The highest shoot induction and multiplication (100% and multiplication coefficient of 10.0) were achieved with KIN (2 mg L−1). Rooting was most successful on MS medium supplemented with α-naphthaleneacetic acid (NAA) (1 mg L−1) and activated charcoal (AC) (1 mg L−1), resulting in 100% rooting. Acclimatized plantlets showed an 80% survival rate under ex vitro conditions. This study presents the first efficient micropropagation system for H. aucheri, contributing to its ex situ conservation and providing a foundation for future pharmacological and biotechnological research.